This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Download PDF. 0´ 104 1. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Note: Additional readers with similar performance are listed. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Danaher Inc spectramax i3x. Align the two slots on the bottle holder with the two pegs on the rail. 1 software Download page. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Unlimited breadth of. 312 gain calibration failed. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Both readers feature an 8-channel read head for fast reads and. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. ”Figure 3: Sensitivity of the SpectraMax i3x reader in detecting increasing dilutions of the MycoAlert Assay Control. It allows you to explore cellular pathways and protein activation and expression in one system. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. 800. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. The calculated LOQ values were 0. 7 Figures from 1126 Publications Promega. Molecular Devices Software. 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). Determination of anti-inflammatory. . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 306 plate air cal fail,low light. Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Description 1. Register to download your free eBook today. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. 9000-0756. Amplitude : 1. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Open the catalog to page 2. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Note: Additional readers with similar performance are listed. 3. The assay can accurately test the toxicology of compounds as shown in Figure 1. It can be upgraded with. MitoXpress-Xtra Oxygen Consumption Assay - Agilent. Unplug the power cable from the back of the instrument. 305 reference level saturation. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors; It allows you to explore cellular pathways and protein activation and expression in one system;In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. 6% for the on-board optics (monochromators). Learn more about its features, specifications, and applications in this user guide. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. Pick the best speed, sensitivity, and price for your needs. . 2 x 10-14. 9 x 10-15. • Removing the transport locks from the PMT shutters and drawers. . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® i3x Multi-Mode Microplate Reader evolves with your future needs and offers an unlimited breadth of application possibilities. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. infoboxeu@moldev. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. pathways and protein activation and expression in one system. SoftMax Pro. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. SpectraMax i3x Multi-Mode Microplate Reader. Bioz Stars score: 98/100, based on 1 PubMed citations. 1 Microplate Data Acquisition & Analysis Software for Windows. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Curcumin binding assay. Read modes. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Description 1. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. Explore a wealth of applications with the future-ready SpectraMax i3x. Find regional support. We would like to show you a description here but the site won’t allow us. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. each new assay. Find regional support. Bulk Kit. 1. Also refer to this Multimode Reader Comparison Chart. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. . Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Controls containing all reaction. Restart the system. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. 100 fmol 1536-well (8 µL) 0. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. The corrected absorbance is within 5% of the. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 3. The Results. Expanded dynamic range Engineered for performance with spectral fusion illumination for increased sensitivity across the entire excitation range. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformVersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Catalog excerpts. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . Examples: “Press carefully to engage,” “ Do not press down. Microplate Reader Shelf. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. i3x. Actual shake speed is based on the microplate format. Version 7. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. The Curcumin binding to p53Y220C was also measured using the emission maxima of Curcumin at 495 nm after. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. techsupport. . china@moldev. We would like to show you a description here but the site won’t allow us. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. Order Now. Controls containing all reaction. Danaher Inc spectramax i3x. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. More Product Information. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Molecular Devices. *On the SpectraMax i3x reader only. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. . Spectramax I3x, supplied by Danaher Inc, used in various techniques. . Expanded dynamic range The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. 7. 22 μ m fi ltration. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. Modularity does provide some measure of forward vision into upcoming or future experimental needs. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). VAL014, right table) assays on the SpectraMax i3x reader are shown below. Results were plotted as ratio of green/red vs. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. We would like to show you a description here but the site won’t allow us. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 99 to $1999. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. HTRF IP standard curve. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The SoftMax Pro Software provides integrated instrument control. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. com. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringLuciferase activity was measured using a SpectraMax i3x microplate reader (Molecular Devices). 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. Published Dec 16, 2020 | Updated Apr 13, 2023. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. 304 signal level saturation. Structured Review. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax i3x and iD5 readers can be equipped with an HTRF detection cartridge or Enhanced TRF module and filters, respectively. 1. SpectraMax Microplate Readers. 003 fmol 0. Average 98 stars, based on 1 article reviews. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. ü; ü. 4. The plate was covered and incubated for one hour at room temperature. The SpectraMax® i3x reader allows you to. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. 2 Parallel Line Analysis Conc log [ng/ML] RLU. 5 Figures from 202 Publications Thermo Fisher Scientific. 315 can't find zero order. 58% for the detection module and 2. Get a front row view of your cells. 58% for the detection module and 2. These robust readers have been placed in labs from Antarctica to the. HeLa cells were plated at 15,000 cells per well in 100 µL. In this product video, see how the SpectraMax M5 quickly reads samples. 5577 Web: Email: info@moldev. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. *On the SpectraMax i3x reader only. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. . Download PDF. 1. Contact Local Team. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Summary of Contents for Molecular Devices SpectraMax M2. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. SoftMax Pro. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. ZERO BIAS - scores, article reviews, protocol conditions and more. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). 635. A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. Engineered for high performance, the auto dual injector cartridge includes fail safe features. com Check our website for a current listing of worldwide distributors. Register to download your free eBook today. +1 800-635-5577. Capture Flash Assays with Ease. Products : SpectraMax i3x reader, SoftMax Pro GxP Software, SpectraMax i3, SoftMax Pro Software, SpectraMax MiniMax cytometer When I attempt a read with my SpectraMax i3 or i3x reader using the injector cartridge, why do I see a plate height error, even though I have entered the correct plate height?. SeeSpectraMax MiniMaxImagingCytometeronpage85. Viewing this answer requires an authorized account. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax i3x and Paradigm readers require the FP-FLUO. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. View the Molecular Devices website. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Contact Us Phone: +1. Background Parallax,Columns 2. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. . It allows you to explore cellular . SpectraMax ® i3/i3x Multi-Mode Microplate Reader; SpectraMax Plus 384 Absorbance Microplate Reader; Methods. The protocols are also available on the SoftMax Pro Software protocol sharing. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Not compatible with Home Edition. Monday to Friday 7am - 5pm Pacific Time. com. Get a front row view of your cells. . 5. 7 mm for all shake intensities. The CellTiter-Glo assay from Promega uses luciferase. . Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Danaher Inc spectramax i3x. 0. 0 mm hex key. The plate was shaken and incubated in the dark for 10 minutes. Ease of use. 50: $75: Improving patient lives by advancing the field of resuscitation medicine. SpectraMaxMiniMaxImagingCytometerInstallationGuide 10 5024111D AttachingtheCytometertotheMicroplateReader Prepareaworkareathatisapproximately130 cm(51. The SpectraMax i2X is used for cell migration, cell. 01 0. Find our technical support information along with contact details. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. ImageXpress Confocal HT. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. 302 low ref light. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. Figure 4. pH-Xtra Glycolysis Assay - Agilent. 314 low lamp level warning. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. See Unpacking the SpectraMax Paradigm Instrument on page 1. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 313 reference gain check fail. The SpectraMax i3x reader allows one to unravel the mysteries of science by exploring cellular pathways and protein. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. SpectraMax® Microplate Readers and SoftMax® Pro Software. 020 fmol 0. Winnersh Triangle. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. 99 to $1999. Figure 1. spectramax i3x - by Bioz Stars , 2023-11. 0´ 104 4. Bioz Stars score: 98/100, based on 1 PubMed citations. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. HTRF-compatible SpectraMax Multi-Mode Readers. . 2. 98 / 100 stars. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Molecular Devicesの全商品を見る. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. 1. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Read height should be optimized for . 3) At the bottom of the strip, click on "Show. )wideand65. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. user upgradeable. . Download SoftMax® Pro 7. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). SpectraMax i3x, onboard luminescence 121. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. The treatments were. 001 0. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. g. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. The dynamic range of the assay is about 3. . Introduction. Our absorbance plate readers feature our PathCheck Sensor. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Description 1. i3x Multi-Mode Microplate Reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. . For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. .